Alcohol content in Wine
With this test is possible to determine alcohol in wine, which represents an important qualitative parameter.
Instruments to Use
WineLab is simple and reliable system to perform analysis of Wine.
The system is supplied pre-calibrated and ready for use. Results are expressed in accordance with the reference method. It is also possible to standardize the system using samples with a known titration.
Test method
End Point test
Colorimetric reaction at 366 nm
Testing time: 5 minute - 30 test/hour
It is possible to carry out several test sessions with a maximum of 14 samples.
Calibration can be performed by aligning the test to reference values.
Test principle
Ethanol is oxidized in an alkaline environment through an enzymatic reaction and converted in acetic acid in presence of NAD, which in turns produces NADH. The increase of the absorbance, measured at 366 nm (end-point) is directly proportional to the concentration of ethanol in the sample.
Sample
Wine as is
Using WineLab you can also perform
- Total Acidity in Wine
- Acetic Acid (Volatile Acidity) in Wine
- Free sulfur dioxide (Free SO2)
- Total sulfur dioxide (Total SO2)
- Malic acid
- Lactic acid
- pH
- Sugars in wine and must
- Anthocyanins in wine and must
- Polyphenols
- Colour intensity and tonality
Reagent Kits
The kit, designed for 10 tests, comprises:
- R1: package with 10 pre-filled cuvettes with buffer.
- R1a: bottle with NAD+ in buffer TRIS/HCl.
- R2: bottle with lyophilized powder (2 bottles in each package).
- Starter R3: bottle with enzymatic solution.
- 10 cuvettes with solvent to dilute sample.
- 1 bottle with reconstituent buffer for R2
| KIT CODE | SAMPLE VOLUME | RANGE |
| *300280 | 50 uL diluted | 4 – 22 %vol |
| Reagent developed by CDR s.r.l | ||||
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Quality System certified ISO 9001 Ed. 2008 |
