Free Fatty acids (FFA) in oil and fat
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Acidity value of food fat is determined by the quantity of free fats derived by the hydrolytic rancidity of the triglycerides. This alteration happens when the alimentary fats are not stored or treated correctly; hence, this test gives a fundamental index of the genuineness of the product.
This chemical test, used to determine the acidity value, is particularly important for fat and oil refining process, for controls during the working cycle and to define the correct good category to which the substance belongs to.
Download pdf datasheet Free Fatty acids (FFA) analysis on oils and fats
Instrument
Reagents
R (in cuvette): alcohol mixture with potash, phenophtaleine derivative
Test method
Test type: End Point
Colorimetric reaction at 630 nm
Testing time: 3-6 minutes
It is possible to carry out several test sessions with a maximum of 14 samples.
Calibration can be performed by aligning the test to reference values.
Test principle
Sample fatty acids, at a pH < 7.0, react with a chromogen developing a colour with an optical density that, if measured at 630 nm, is proportional to the concentration of the fatty acid, expressed as percentage of oleic acid. CDR innovative method simplifies and speeds up the standard procedure. It is possible to carry out a test using micro-quantities of sample and can be applied even to fat substances that have a difficult extraction process.
Sample
Oil as is (extracted from olive, seeds, nuts, etc.).
Solid fats: melted using bain-marie method before starting the test (animal fats, palm oil, etc.).
Extractable fats: extract the fatty part following the instructions provided in the test method (butter, cream, margarine, semi-finished products, etc.).
Kit
The reagents are provided in sealed bags, each containing 10 test tubes. They have a 12 months duration, starting from the production date. Stocking requirements are described on the bag label.
Reagents do not contain dangerous solvents and are ready for use.
| CODICE KIT | VOLUME CAMPIONE | RANGE |
| *300128 (10 test) *300125 (100 test) |
1 – 10 µL | 0,01 – 25 % oleic acid |
Datasheet
