Determination of the glucose concentration in tomato (fresh tomato, pulp, concentrated tomato, etc.)

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Glucose in tomato

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  • Glucose in tomato

The chemical test for determining the concentration of glucose, fructose and total reductive sugars it is used to quantify the sugar present in fresh tomato, tomato sauce and tomato concentrated. Unlike traditional methods that requires a specific treatment of the sample this method uses the sample as is; it is very easy to carry out and can be done directly in production line or at the raw material acceptance desk.

Instrument

FoodLab

Reagents

R1 (pre filled in test tube): Pipes - phenolic derivatives
R2 (in dropper):  GOD - POD - 4-aminoantiphirine

Test method

Test type: End Point
Colour reading at 545 nm   
Testing time: 3 minutes
It is possible to carry out several test sessions with a maximum of 14 samples.
Calibration can be performed by aligning the test to reference values.

Test principle

In presence of the peroxidase, glucose reacts with a phenolic derivative and forms a purple complex whose intensity, measured at 545 nm, is directly proportional to the concentration of glucose in the sample. From the value of glucose, using a mathematical calculation, it is automatically displayed to the concentration of fructose and reductive sugars contained in the samples.

Sample

Tomato (fresh, pulp, concentrate, etc.) diluted in distilled water.

Kit

The reagents are provided in sealed bags, each containing 10 test tubes. They have a 12 months duration, starting from the production date. Stocking requirements are described on the bag label.
Reagents do not contain dangerous solvents and are ready for use.

Immagine Glucose in tomato
CODICE KIT VOLUME CAMPIONE RANGE
*300450 (10 test)
*300451 (100 test)
5% µl 0,01 - 10 g/100 g glucose