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p-Anisidine value (AnV) in oils and fats

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  • p-Anisidine value (AnV) in oils and fats

Using FoodlabFat you can carry out a chemical test for the determination of p-anisidine value (AV) of oils and edible fats, using micro quantity of sample and a fast, simple and realiable method.

P-anisidine value (AnV) play an important rule in the oxidation process of edible oil and edible fats. Infact the complex oxidation process of an oil on a fat can be split into two phases. In the first phase the fatty acids react with oxygen forming odorless compounds as peroxides. In the second phase, peroxides decompose and develop other substances as aldehydes, which are responsible for the rancid smell and taste.  The chemical test used for determination of p-Anisidine value content in edible fats and edible oils permits the detection of a parameter correlated to the presence of aldehydes derived from the secondary oxidation of fat substances.

Furthermore p-anisidine test on oil is an indicators of excessive oil deterioration in deep frying process

Request more information about p-anisidine value analysis in oils and edible fats: click and contact us

Instrument

FoodLabFat

Reagents

R (in cuvette): alcohol mixture, p-anisidine

Test method

Test type: kinetic
Colorimetric reaction at 366 nm   
Testing time: 2 min
Calibration can be performed by aligning the test to reference values.

Test principle

Aldehydes deriving from the secondary oxidation of fatty substances react with p-anisidine determining an absorption that can be measured at 366 nm . The p-Anisidine value is expressed as AnV (Anisidine value).
CDR innovative method simplifies the standard AOCS (Cd 18-90) procedure since permit to test micro-quantities of samples (melted oil or fat) without any treatment and uses a mono reagent pre-filled into a disposable cuvette.

Sample

Oil as is (olive, seed, nut oil, etc.).
Solid fats: melted with bain-marie before collection (animal fats, palm oil, etc.).
Extractable fats: extract the fatty part following the instructions provided in the test  method ( butter, cream, margarine, semi-finished products, etc.).

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Kit

The reagents to determine AnV are provided in sealed bags, each containing 10 test tubes. They have a 12 months duration, starting from the production date. Stocking requirements are described on the bag label.
Reagents do not contain dangerous solvents and are ready for use.

CODICE KIT VOLUME CAMPIONE RANGE
*300503 (10 test)
*300500(100 test)
20 µl 0,5 - 100 AnV