Soaps in oil and fat
 |  |
Soaps are salts of fatty acids obtained from a reaction that occurs between free fatty acids and sodium carbonate. Thanks to their water solubility and insolubility in oil, they can be separated from fat through specific procedures. Alimentary industries use this technique to lower fat acidity and to enhance its organoleptic characteristics. For such industries, it is important to carry out a chemical test for determining the concentration of soaps in oil and fat.
Instrument
Reagents
R1 (in cuvette): alcoholic mixture
R2 (in vial): cromogen
Test method
Test type: End Point
Colorimetric reaction at 604 nm
Testing time: immediate
It is possible to carry out several test sessions with a maximum of 14 samples.
Calibration can be performed by aligning the test to reference values.
Test principle
At specific pH values soaps mixed with bromphenol, generate a specific blue colour. Its intensity, measured at 604 nm, is directly proportional to the concentration of soaps in the sample. CDR innovative method simplifies and accelerates the standard procedure, because it enables the test to be carried with micro-quantities and to be applied to fats that are difficult to extract.
Sample
Oil as is (extracted from olive, seeds, nuts, etc.).
Solid fats: melted using bain-marie method before starting the test (animal fats, palm oil, etc.).
Extractable fats: extract the fatty part following the instructions provided in the test method ( butter, cream, margarine, semi-finished products, etc.).
Kit
The reagents are provided in sealed bags, each containing 10 test tubes. They have a 12 months duration, starting from the production date. Stocking requirements are described on the bag label.
Reagents do not contain dangerous solvents and are ready for use.
| CODICE KIT | VOLUME CAMPIONE | RANGE |
| *300175 (100 Test) *300179 (10 Test) |
5 – 50 µL (it changes with test sensitivity) | 5 – 1350 ppM |
